The BreaKERs – developing bacterial keratinase expression to degrade human hair and chicken feathers
2015 TFC Teams (formerly geekStarter)
Our Lady of the Snows Catholic Academy (Canmore)
The BreaKERs – our project aims to break down hair in wastewater treatment facilities, and to break down feather waste in the poultry industry
|Team Grade Level:
|| Synthetic Biology
Optimizing keratinase expression (keritinase is an enzyme that breaks down keratin which is structural protein used in hair, nails, fur and feathers) provides a great opportunity to impact the management of the estimated 8.5 billion tons of poultry feather from farms globally as well as the tons of human hair clogging up waste water treatment facilities worldwide. Also, bio-degraded keratin from chicken feathers has been shown to be useful for commodity products such as fertilizers and livestock feeds. Our team is developing two keratinase-producing E. coli bacteria using the KERA and KERUS genetic sequences found naturally in the Bacillus genera. The KERA and KERUS sequences will be optimized for expression in E. coli, synthesized into plasmid rings, and ligated into a standard biobrick backbone for submission to the iGEM parts registry. An IPTG-inducible promotor (part BBa_J04500) from the standard registry of parts will then be added to each KER gene to express this protein.
iGEM Medal Achieved: Bronze